Richard “Torpedo” Thompson, a former Olympic 100-metre silver medallist, would not be on the podium for that event at Rio.
Thompson did a time of 10:29 and failed to make it to the 100-metre final.
The 31-year-old T&T sprinter, who has the ninth best 100-metre performance of all time with 9:82, was barely eclipsed as one of the fastest non-automatic losers.
American Justin Gatlin did 10:01 to win his heat and do the fastest time in the semi-final races.
Jamaican Usain Bolt comfortably won his heat in 10:07 and would also contest the final.
Bolt remains on course to become the first athlete in history to win the 100-metre event at three successive Olympic Games.
Thompson, who is coached by former Olympic sprinter Ato Boldon, won silver in the 100-metre event at the 2008 Beijing Olympics.
He was also a member of the quartet which won bronze in the 4 X100-metre event at the London 2012 Olympics.